Csfv e2 phosphatase
WebSep 1, 2005 · APCs transfected with either of the mRNA molecules restimulated CSFV-specific T cells to produce gamma interferon and specific cytotoxic activity against CSFV-infected target cells. The presence of CTL epitopes on E2 was confirmed by using d/d-haplotype MAX cells expressing E2 constitutively as target cells in d/d-haplotype CTL … WebRecombinded CSFV E2 protein; classical swine fever virus (strain Alfort) (CSFV) (Hog cholera virus) 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. …
Csfv e2 phosphatase
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Webwe identified protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a specific binding host partner for E2. We further confirmed the occurrence of this interaction in CSFV-infected swine cells by using two independent methodologies: Co-immunoprecipitation and Proximity Ligation Assay. WebThe recombinant CSFV E2 sequence was also fused to a 6× histidine tag to facilitate purification and the endoplasmic reticulum (ER) retrieval HDEL tag to allow accumulation of the protein in the endoplasmic reticulum. All genes were codon optimized for plant expression using the Nicotiana tabaccum codon usage table and synthesized by Eurofins ...
WebJan 8, 2014 · E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry … WebThe CSFV E2 protein has been widely used as a major antigen for antibody detection. It is important to improve the affinity between the E2 protein and CSFV antibodies to improve the performance of the detection method. In this study, a recombinant E2 extracellular protein (amino acids 1–331) with a native homodimer conformation and high ...
WebMar 15, 2024 · A series of truncated and overlapping fragments of CSFV E2, shown in Fig. 3, were designed in this study, and the related primers were listed in Table 1.These Truncated E2 genes were cloned into the expression vector pET-30a. After confirmation in DNA sequencing, positive plasmids were transformed into Escherichia coli BL21 (DE3) … Web丁香通为您提供蚂蚁淘代理品牌FabGe商品详情介绍：价格：￥5600，货号：PD4B3-221AP，品牌：FabGennix，详见丁香通蚂蚁淘代理品牌FabGe商品详情页；
Webwe identified protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a specific binding host partner for E2. We …
WebMay 1, 2014 · Monoclonal anti-E2 CSFV antibody (diluted 1:1000) and rabbit polyclonal serum anti-E rns CSFV (diluted 1:250) were used as primary antibodies. Anti-mouse and anti-rabbit alkaline phosphatase (AP)-conjugated antibodies were used as secondary antibodies (diluted 1:2000). Antigen-antibody complexes were detected using NBT and … ray tracing schede videoWebMar 12, 2024 · CSFV E2, CHO-pTxnip-4-rE2 cell clone (F12), was des-ignated as the target cell clone for recombinant E2 protein production in suspension cultured mode. CHO-pCMV-rE2 (A11) and parental CHO cells were conducted the same process as control. In 6 days batch-wise suspension culture performed in 1 L shaking flask with 550 mL SFM, recom- simply piano ad lyricsWebOverall, our data suggests that the CSFV E2 and PPP1CB protein interact in infected cells, and that activation of the PP1 pathway decreases virus replication. simply piano app for windows 10WebApr 9, 2024 · E2 is the major structural glycoprotein of the classical swine fever virus (CSFV). E2 has been shown to be involved in important virus functions such as replication and virulence in swine. Using the yeast two-hybrid system, we previously identified several host proteins specifically interacting with CSFV E2. Here, we analyze the protein … simply piano 50% offWebMar 15, 2024 · A series of truncated and overlapping fragments of CSFV E2, shown in Fig. 3, were designed in this study, and the related primers were listed in Table 1.These … simply piano contact numberWebDec 5, 2005 · CSFV E1 (B) was detected with an E1 specific rabbit antiserum, and CSFV E2 (C) was detected with mAb WH303. ... slides were incubated with alkaline-phosphatase-conjugated, goat anti-mouse antibody for 20 min at room temperature. Acknowledgments. We thank Aniko Zsak, Adriene Lakowitz, and the PIADC animal care staff for excellent … simply piano apk crackedWebDec 12, 2024 · The E2 protein in classical swine fever (CSF) virus (CSFV) is the major virus structural glycoprotein and is an essential component of the viral particle. E2 … simply piano app for fire