WebThere are two procedures commonly used for acid-fast staining: • Carbolfuchsin methods which use Ziehl-Neelsen and Kinyoun methods (direct microscopy) • Fluorochrome procedure using auramine-O or auramine- rhodamine dyes (fluorescent microscopy) WebAcid-fast staining is another commonly used, differential staining technique that can be an important diagnostic tool. An acid-fast stain is able to differentiate two types of gram-positive cells: those that have waxy mycolic acids in their cell walls, and those that do not.

AURAMINE O STAIN - med-chem.com

WebIn the fluorochrome acid-fast stain, the reagent used to quench background fluorescence is. potassium permanganate. A catalase-positive, gram-positive bacillus that is not acid-fast, does not branch, and does not form spores could possibly belong to which group of bacteria? ... fluorescent acid-fast stain (auramine O with or without a second ... WebFluorochrome acid -fast stain i. Auramine O ii. 0.5% Acid -alcohol iii. Counterstain (potassium permanganate or acridine orange) 2 Auramine O Stain Protocol B. Supplies a. Glass slides (25 by 75 mm), frosted ends desirable b. Coverslips (22 by 22 mm; no. 1) c. Sterile 50 -ml conical polypropylene screw -cap tubes (aerosol free ... ear tube drainage yellow

WEEK 12: Self Assessment (Myobacterium) Flashcards Quizlet

WebMar 18, 2009 · Summary of fluorochrome acid-fast stain results Open in a separate window aMicroscopy results were quantified using the CDC guidelines (4) as follows: negative, no AFB or 1 or 2 per slide seen at a magnification of ×250; 1+ positive, 1 to 9 AFB/10 fields; 2+ positive, 1 to 9 AFB/field; and 3+ positive, ≥10 AFB/field. WebOf the different types of staining and microscopic techniques, which one of the following is basically the most sensitive? Fluorescence The fluorochrome stain that can be used to … WebThe Kinyoun method can be modified as a weak acid fast stain, which uses 5% sulfuric acid instead of hydrochloric acid. ... In some cases, mycobacteria can be stained by Gomori methenamine silver stain or periodic acid–Schiff stain. Fluorochrome stains are more sensitive, though not as specific, but may be useful for screening of the tissue ... cts eventim maut