Splet01. jul. 2012 · The Cre expression construct, pBS513 EF1alpha-cre, was purchased from Addgene (Cambridge, MA). RNA ISOLATION AND cDNA SYNTHESIS. Total cellular RNA was harvested from either cortical bone or culture cells using QIAzol Lysis Reagent and RNeasy Mini Columns (Qiagen, Valencia, CA). DNase treatment was performed to degrade … SpletHover: Reveals additional information for enzyme cut sites, ORFs, feature descriptions, and primer details. Select: Click one location on a nucleotide and then click on a second …

Dual RMCE for efficient re-engineering of mouse mutant alleles

http://www.biofeng.com/adjy/zhili/pF155%20pcDNA3.1(+)SOD1G93A.html Splet17. okt. 2010 · The human EF1A promoter was later inserted 5′ as a HindIII-BamHI fragment derived from the pBS513 EF1alpha-Cre plasmid (Addgene). The SV40 polyadenylation … how to replace an exterior door handle

Sequence Analyzer: pBS513 EF1alpha-cre Sequencing Result

SpletAddgene inc pbs513 ef1alpha cre Pbs513 Ef1alpha Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 SpletThe considerable potential of Cre recombinase as a tool for in vivo fate-mapping studies depends on the availability of reliable reporter mice. By targeting a tandem-dimer red fluorescent protein (tdRFP) with advanced spectral and biological properties into the ubiquitously expressed ROSA26 locus of C57BL/6-ES cells, we have generated a novel ... how to replace an exterior door jamb