Phospho flow antibodies

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August undefined, 2024

WebPhospho-SMC1A-S957 Polyclonal Antibody WebAll lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate Lane 2 : Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate Lane 3 : Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 µg. Then the membrane was incubated with …

Flow Cytometric Analysis of STAT Phosphorylation

WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization, Foxp3/Transcription Factor Buffer, and IC Fixation Buffer/Methanol. The recommended buffer system(s) will be noted on the … Find manuals and technical documents, get account and ordering help, browse … Flow Cytometry Support Center—Find technical support recommendations for … The Foxp3 Transcription Factor Staining Buffer Set has been formulated and … WebThis Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033. Product Usage Information Storage hi hand drawing

Recombinant Anti-AMPK alpha 1 (phospho T183)

WebRabbit recombinant monoclonal p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] conjugated to PE. Validated in Flow Cyt and tested in Rat. Immunogen corresponding to synthetic peptide. WebApr 9, 2024 · “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation” The idea is that the antibody is: ‘pan’ in that it will react with all phospho-histidine residues on proteins, but ‘specific’ in that it will not react with unphosphorylated histidine residues or other phospho-aminoacids e.g. phospho-serine. Share WebPhospho-NFkB p65 (Ser536) Antibody Applications: WB, FCM, ICC, IP Reactivity: Hu, Ms, Rt, Ca, Hm, NHPr, Pg Conjugate/Tag: Unconjugated Quantity: 100 µL Citations: (25) Supplier Page Sign In or Register to view pricing Compare Product + See More (4) View All (18) Biorbyt NFKB p65 antibody Applications: WB, ELISA, FCM, ICC, IF, IHC-fr, IHC-p hi happy iguatemi

Anti-p65 Flow Cytometry Antibody Products Biocompare

WebAliquot an optimal concentration of phosphospecific fluorochrome-conjugated antibody to each tube. Incubate at room temperature for 30 minutes in the dark. Wash once with 2 mL … WebPE anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody - ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ... Each lot of this … hi hat 140 loopermanWebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. hi happy shopping cerrado

"WebPolyclonal Antibody for studying PLCg1 (Tyr783) phosphate. Cited in 265 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously … " - Phospho flow antibodies

Phospho flow antibodies

Recombinant Anti-AMPK alpha 1 (phospho T183)

WebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody. WebTermed "phospho-flow," these methods employ fluorescence-conjugated monoclonal antibodies that recognize phosphorylated epitopes of intracellular kinases, and may be combined with surface phenotypic markers to observe changes in …

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WebAnti-RhoA (phospho S188) antibody See all RhoA primary antibodies Description Rabbit polyclonal to RhoA (phospho S188) Host species Rabbit Specificity Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins. Tested applications Suitable for: WB more details Species reactivity Reacts with: Human WebPhospho-flow has been applied to numerous areas of biology, including antigenic stimulation and microbial challenge, high-throughput and high-content drug discovery, …

WebPhospho-specific antibody flow cytometric analysis of phosphorylated proteins allows researchers a quick and effective way to measure signaling cascades in individual cells. A … WebPhosphoflow is a specialized application of flow cytometry that enables researchers to examine the phosphorylation status of specific proteins that govern cellular responses …

WebPrimary Anti-ULK1 (phospho S556) antibody (ab203207) Primary Anti-AMPK gamma 1 antibody [Y308] (ab32508) Primary Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) View more … WebPhosphoserine Antibodies Antibodies that detect Phosphoserine can be used in several scientific applications, including Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation and Dot blot. Our Phosphoserine monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. Find the Phosphoserine antibody that fits …

WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship.

WebPhospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb detects endogenous levels of Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. Species Reactivity: Human, Mouse, Rat Source / Purification hi happy halloweenWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. hi hard radioWebFlow Cytometry - APC Anti-p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] (ab278728) Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278728. hi happy wednesdayWebAnti-Phosphoserine antibody See all Phosphoserine primary antibodies Description Rabbit polyclonal to Phosphoserine Host species Rabbit Specificity Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. hi hat 100mm bowlWebAnti-p65 Flow Cytometry Antibody Products. Products (449) User Reviews (21) Company View. Product View. Your search returned 449 p65 Flow Cytometry Antibodies across 28 … hi happy wednesday imagesWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free. Suitable for: ELISA, Dot blot, WB, IHC-P. Reacts with: Human. hi hascoWebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. hi hat and snare how do they go on abelton