Stat5 reporter assay

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August undefined, 2024

Web6.2 STAT5 Signaling. STAT5 was shown to be a mediator of erythropoietin-induced neuroprotection in a rat model of global cerebral ischemia (Zhang, Wang, Cao, Gao, & … WebFeb 2, 2024 · Two duplicate plates were set up, one for SEAP reporter assay and one for CellTiter-Glo cell viability assay. SEAP was determined using QUANTI-Blue (InvivoGen, rep-qbs).

Biology Free Full-Text STAT5-Interacting Proteins: A Synopsis of …

WebFeb 2, 2024 · STAT5 is a key member of the JAK/STAT core cancer pathway, activated by a plethora of cytokines and growth factors to regulate a wide spectrum of physiologic … WebWe established and validated a rapid, sensitive, flow cytometric based pSTAT5A assay to detect T cell proliferation. We showed that there was a strong correlation between the early CD3/CD28 or polyclonal mitogen … mary berg vegan recipes

STAT5 Reporter Assay Compound Screening Services - Abeomics

WebIn an attempt to use the cells for large-scale chemical screens to identify JAK3 inhibitors, we established a cell line, 32D/IL-2Rβ/6xSTAT5, stably expressing a STAT5 reporter gene. Treatment of this cell line with IL-2 or IL-3 dramatically increased the reporter activity in a high-throughput format. WebReporter Vector primer 4 (RVprimer4) binding region 4171–4190 Sequence information for the pGL4 Vectors is available online at: www.promega.com/vectors/ Example Protocol In … mary berg vegetable nachos

A cellular reporter assay to monitor insulin receptor kinase activity ...

STAT5 Reporter (Luc) – Ba/F3 Cell line - BPS Bioscience

WebUpon IL-2 or IL-15 stimulation, levels of STAT5-induced SEAP can be readily monitored using QUANTI-Blue™ Solution. HEK-Blue™ IL-2 cells and HEK-Blue™ CD122/CD132 cells … WebOct 23, 2024 · Reporter assays demonstrated that Shd1 repressed Stat5-mediated transcription in a dose-dependent manner without affecting Stat5 stability or phosphorylation. Nakajima et al. (2009) concluded that SHD1 is a cytokine-inducible negative-feedback regulator of STAT5. huntley napa west unionWebLike the Kinase receptor activation (KIRA) assay, developed by Sadick et al. [1], the STAT5 phosphorylation assay was performed using two separate microtiter plates: the first one … mary berg wedding photos

"WebSTAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling … " - Stat5 reporter assay

Stat5 reporter assay

WebAug 3, 2016 · STAT5 deficiency prevented IL-3-mediated inhibition of osteoclastogenesis, suggesting a key role of STAT5 in IL-3-mediated inhibition of osteoclast differentiation. In … WebThe STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … Recombinant Sortase A, His-tag (BPS Bioscience #71086) dose-dependent …

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WebThis assay utilized the growth factor-dependent human erythroleukemia TF1 cell line engineered to express a green fluorescent protein-STAT5 fusion protein. Furthermore, a complementary beta-lactamase reporter gene assay was developed to analyze the transcriptional activity of STAT5 downstream of JAK2 in TF1 cells. WebMar 11, 2024 · There is evidence from cyclin D1-luciferase reporter assays that STAT5 binds at -481 bp of the cyclin D1 promoter . Oct-1 has also been shown to have a role in the transcriptional regulation of cyclin D1. Oct-1 (Octamer-Binding Protein 1) is a transcription factor ubiquitously expressed in the nucleus that contains a POU ...

WebJan 27, 2003 · As Dex was used in the STAT5 reporter assay to enhance response , we examined whether the effect of E 2 depended on the presence of glucocorticoid. GH-induced STAT5 activity was significantly lower in the absence than the presence of 250 nM Dex (9.1 ± 0.6-fold and 19.6 ± 1.0-fold, respectively; P 2 without or with Dex (55 ± 2% and 58 ± 1% ... WebA highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed.

WebJan 30, 2024 · We describe here the development and validation of a reporter gene assay (RGA), based on an IL-5-dependent TF-1 cell line variant we established that stably … WebFeb 24, 2024 · General strategy for the design of the reporter cell line: [a] signal through most of the immunologically relevant receptors results in the activation of NF-κB 23, 30, …

WebMay 12, 2010 · A skewed ratio of pro-inflammatory to anti-inflammatory cytokines, elevated growth factor synthesis and T- and B-lymphocyte activation are 3 hallmarks of rheumatoid arthritis (RA) pathology. Interleukin-6 (IL-6), IL-7, IL-17, IL-12/IL-23 and growth factors, granulocyte macrophage-colony stimulating factor, IL-3, and erythropoietin activate the …

WebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK … huntley national night outWebMoreover, bioinformatics analyses and luciferase reporter assay indicated that exosomal miR-182-5p was bound to PPARG 3' UTR sequence and inhibited the expression of PPARG. Collectively, our findings provided a new role of EPCs-EXO in the clinical treatment of diabetic skin wounds. ... (STAT5) to control EPC cell-cycle progression 44. In ... mary berg vegetarian chiliWebThe STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by … huntley newsletterWebHygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Choose a response element ISRE SBE SIE STAT5 RE Size 20µg Catalog number selected: E4041 $ 435.00 Your price: Log in Add to Cart Add to Helix Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors … huntley ndpWebAssay Interferences Interference testing should be relevant to the patient population that will be tested with a given assay endogenous substances - highest reported clinically relevant … huntley museumWebMay 25, 2000 · The finding that glucocorticoid receptor (GR) is associated with Stat5 came from studies on the β-casein gene. Upon interaction with its steroid ligand, GR binds to specific DNA sequence, the... huntley new homesWebAssay Entry Cell-based terbium phospho-specific activity (stable) Cell State Dividing Cells Detection Method Fluorescent Gene Symbol STAT5A Optimal Response GM-CSF (EC50 = … huntley nash cyclist